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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
| Topic 1: Clinical Chemistry | 20-25% | - Toxicology and liver/kidney function tests
- Carbohydrates, lipids, proteins, and enzymes
- Electrolytes, acid-base balance, and blood gases
- Endocrinology and therapeutic drug monitoring
|
| Topic 2: Immunology and Serology | 5-10% | - Autoimmune and immunodeficiency disorders
- Immune system principles and components
- Infectious disease serology
- Immunologic testing methodologies
|
| Topic 3: Laboratory Operations | 5-10% | - Quality control and quality assurance
- Laboratory mathematics and instrumentation
- Laboratory safety and regulatory compliance
- Pre-analytical, analytical, and post-analytical processes
|
| Topic 4: Urinalysis and Other Body Fluids | 5-10% | - Physical, chemical, and microscopic analysis
- Urine formation and composition
- Cerebrospinal, serous, and synovial fluids
- Semen and amniotic fluid testing
|
| Topic 5: Microbiology | 15-20% | - Specimen collection and culture techniques
- Mycology, parasitology, and virology
- Identification of pathogenic microorganisms
- Bacteriology and antimicrobial susceptibility
|
| Topic 6: Hematology | 20-25% | - Routine and automated hematology procedures
- Red blood cell physiology and disorders
- Hemostasis and coagulation testing
- White blood cell morphology and diseases
|
| Topic 7: Blood Banking (Immunohematology) | 15-20% | - Compatibility testing
- Blood group systems and antigens
- Transfusion reactions and adverse events
- Antibody detection and identification
- Blood components and storage
|
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Question 1
1. A
2. A
3. B
4. A
5. B
6. B
A cardiovascular risk marker is an analyte in a body fluid that can be measured by the clinical laboratory and has been associated with the development of cardiovascular disease. Examples of risk markers include:
LDL-C, triglycerides, and hs-CRP.
A cardiovascular risk factor is a condition (not a laboratory analyte) that is associated with an increased risk of developing cardiovascular disease. Examples of risk factors include: smoking, obesity, diabetes and hypertension.
Determine if each of the following is a cardiovascular risk marker or a cardiovascular risk factor.
1. Smoking
2. Obesity
3. Low density lipoprotein (LDL)
4. Hypertension
5. Triglycerides
6. High sensitivity-C-reactive protein (hsCRP)
A. Cardiovascular risk marker
B. Cardiovascular risk factor
Question 2
Myoglobin rises typically within 2-4 hours after a myocardial infarction and stay elevated for days, with a peak at the 6-9-hour mark post-MI. After myoglobin, troponin is the next fastest to rise afer a mycardial infarction and can rise within 3-4 hours with a peak around 12 hours post-MI. LD (8-12 hours, 24-48 hour peak) and CK (4-6 hours with peak at 24-36 hours) will also rise after MI, but are not as fast to rise as myoglobin and troponin.
Chemistry
A patient is admitted to the hospital with acute chest pain, but which of the following cardiac markers will be elevated FIRST if the patient had an MI?
A. Myoglobin
B. LD
C. CK
D. Troponin
Question 3
Match each of the following definitions associated with heart disease and heart failure to the term that it defines.
1. Congestive heart failure
2. Infarction
3. Ischemia
4. Angina
A. An area of tissue death that occurs due to lack of oxygen.
B. A left ventricular dysfunction resulting from aging, hypertension, atherosclerosis or muscle damage from an AMI or repeated AMIs.
C. Chest pain caused by inadequate supply of oxygen to heart myocardium.
D. An inadequate blood supply that decreases availability of oxygen.
Question 4
The clue to the identification of the colony seen in the upper frame is the gram stain in the lower frame, in which are seen short, rounded, gram positive bacilli, many of which possess distinct spores. As the colonies grew aerobically, the presence of spores indicates Bacillus species. The colonies are spreading, smooth, yellow-white and non-hemolytic. The lack of hemolysis and the small size of the bacterial cells suggests a species other than Bacillus cereus, the species causing most human infections. The isolate was identified as Bacillus circulans, which is consistent with the gram stain morphology. Clostridium septicum also produces spores; however, this species is an anaerobe and would grow poorly if at all and not produce spores aerobically. Listeria monocytogenes and Lactobacillus species are gram positive bacilli; however, neither of these produce spores.
The colonies seen in the upper frame, grown after 48 hours incubation aerobically at 35°C, and the accompanying gram stain in the lower frame are uncommonly associated with human disease, but have been associated with septicemia, bronchopneumonia, osteomyelitis and other infections, particularly in intravenous drug users. The most likely identification is:

A. Bacillus circulans
B. Clostridium septicum
C. Listeria monocytogenes
D. Lactobacillus species
Question 5
The tube of choice for trace metal analysis is:
A. red
B. royal blue
C. light blue
D. gold
Solutions:
Question 1 Answer: A,B | Question 2 Answer: A | Question 3 Answer: A,B,C,D | Question 4 Answer: A | Question 5 Answer: B |